DETECT THE TOXIC EFFECTS OF IMIDACLOPRID PESTICIDE BY USING LOCAL CHICK EMBRYOS.

Document Type : Original Article

Authors

1 College of Agriculture-University of Baghdad-Iraq

2 Ministry of science and technology

Abstract

he study aimed to detect the side hazard effects of imidacloprid pesticide on living organism.
The aim of this experiment was to study its effectson the survival rate and the level of specific liver enzymes on local chick embryos. Ninety fertile local eggs were used with 40-45gm average weight. Thirty eggs were used to determine the imadocloprid LD50 and it was 12.5mg/egg. Then the other 60 eggs were divided into six treatment groups (10 egg/ treatment) then incubated. Three days later, treatment eggs injected with different concentrations of malathion which was respectively (0.5, 10, 15, 20, 40) mg/egg (0.1ml/egg). After 16 days eggs where broken and the rate of survival embryos were calculated and also liver enzymes (GOT) Glutamic Oxaloacetic Transaminas ,(GPT) Glutamic Pyruvate Transaminase, (ALP) Alkaline Phosphates& (ADA) Adenosine De Aminaseconcentrations.
From the statistical analysis for survival rate and liver enzymes concentrations we found that the rate of survival embryos was increasing significantly P>0.05 as malathion concentration decreased. The first treatment control shows 100% rate of survival, while it was 0% of sixth treatment with 40% mg/egg imadocloprid, we found that the concentration of GOT enzymes was increase significantly P>0.05 in third and fourth treatment respectively when compared with control group which gave the lowest value.
The GPT enzyme was highly increased significantly P>0.05 on the second embryos treatment when compared with the control and the ALP enzyme value was highly increased significantly P>0.05 on second treatments but was decreased in the fifth treatment but it was not the same value as in the control treatment, while the mean values of ADA the immunity enzyme was increasing significantly P>0.05 on control group and the 2nd treatment while it declined in the fifth treatment but it was medium on third and fourth treatments respectively.
We can conclude that the imidacloprid pesticide has direct harmful effect on environment by proved its direct effect on reducing survival rate and caused different levels on liver specific enzymes in local chicken embryos which used in this research as a biological indicator to detect the harmful effects of environment pollution with chemical pesticides.

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