Document Type : Original Article
Authors
1
Department of Poultry Production, Faculty of Agriculture, Damietta University, Damietta, Egypt
2
Department of Animal Production, Faculty of Agriculture, Mansoura University, Egypt
3
Department of Poultry Productions, Faculty of Agriculture, Mansoura University, Egypt.
4
Department of Animal Production, Faculty of Agriculture, Mansoura University, Egypt.
Abstract
The aim of this study was to evaluate the effect of oral administration of doe rabbits with green tea extract polyphenols (GTPs) at different levels on ovarian activity, oocyte quality, in vitro maturation, fertilization and development of oocytes. Total of 12 mature NZW doe rabbits (5-6 months of age, 2.5-3.20 kg LBW) were used in this study. Does were divided into 3 similar groups, (n=4). The 1st group (G1) was control, while does in the 2nd (G2) and 3th (G3) groups were given daily for one month oral 1 ml distilled water containing 200 and 400 mg/kg LBW of GTPs, respectively. At the end of treatment period, does in each group were slaughtered and immediately ovaries were removed and oocytes were collected by slicing technique for evaluation. Only compact-cumulus oocytes (COCs) were in vitro matured and fertilized. Results showed that ovarian weight relative to LBW, oocyte number/doe and recovery rate of oocyte were higher (P<0.05) in G2 and G3 than in G1. Number of visible follicles/doe was not affected by treatment. Frequency distribution was higher (P<0.05) for COCs and lower (P<0.05) for denuded oocytes in G2 and G3 as compared to G1. Frequency distribution of both expanded and partial denuded oocytes were not affected by treatment. Percentage of full expanded oocytes was the highest (P<0.05) in G2, followed by G3, while G1 showed the lowest (P<0.05) values. Percentage of partial expansion oocytes was higher (P<0.05) in G3 than in G1 and G2. Fertilization rate was higher (P<0.05) in G2, followed by G3, while G1 showed the lowest (P<0.05) values. Percentage of blastocysts was higher (P<0.05) in G2 and G1 than in G3. Percentage of morulae, and degenerated and un-cleaved embryos were not affected by GTPs treatment. In conclusion, treatment of doe rabbits with green tea extract at a level of 200 mg/kg LBW as daily oral dose for one month is recommended to enhance ovarian activity, oocyte quality, cytoplasmic maturation and in vitro embryo production.
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